Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring.

نویسندگان

  • M Carmen Galan
  • Anh Tuan Tran
  • Claire Bernard
چکیده

A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.

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عنوان ژورنال:
  • Chemical communications

دوره 46 47  شماره 

صفحات  -

تاریخ انتشار 2010